Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.056298 |
Chromosome: | chromosome 13 |
Location: | 4926765 |
Confidence (%): | 40 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g605550 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCCGGCGCGCGCAGTGCCCCCAGCCCGACCGCGCTGTGGACTGGTCG |
Internal bar code: | TAAGTTTGGCTTGACTCCCATA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 187 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 43 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACGCTTTTCACTGCTGCAG |
Suggested primer 2: | CCTGCAGTGCTGGTACAAGA |