| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056315 |
| Chromosome: | chromosome 6 |
| Location: | 6725555 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTTCTAGTTGAGCAATCCCACTCCGGTCTTCCATTTCTGCTACGGCT |
| Internal bar code: | AATTTGCGGTACCAAGGGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1883 |
| LEAP-Seq percent confirming: | 85.9155 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCACCTGTCGGTCAGAA |
| Suggested primer 2: | ACGTGTGAAAGGCAGCGATA |