| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056318 |
| Chromosome: | chromosome 3 |
| Location: | 7360709 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCCCCGTCCCTACCTGCCGGTGCAGCGACACCCTACCTACCCGGCGT |
| Internal bar code: | AGTCAAAGTTAAAACGGAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1364 |
| LEAP-Seq percent confirming: | 30.5556 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCATGCACTGCGGCTATTTC |
| Suggested primer 2: | GTACACTCGCTCCAAACCCA |