| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.056330 |
| Chromosome: | chromosome 5 |
| Location: | 1366445 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234636 | PFLAE,PFA1,PFLA1 | Pyruvate formate lyase activating enzyme 1; (1 of 1) 1.97.1.4 - [Formate-C-acetyltransferase]-activating enzyme / S-adenosyl-L-methionine-cleaving) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGGCGACTGCGGCCACACCTCGTGACCTGTGACCCCAGCTGAAGTC |
| Internal bar code: | CATGTTTGATGTGCGTTTGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1381 |
| LEAP-Seq percent confirming: | 95.4023 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTTTGGCCTCTCGTGAT |
| Suggested primer 2: | AGAGGACTCGAGCTAGGGTG |