| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.056336 |
| Chromosome: | chromosome 10 |
| Location: | 171758 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418900 | (1 of 18) K13412 - calcium-dependent protein kinase (CPK) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGTTGCCAACGCTATTGACATTGTACTTGAAGATGCAAGACGTTCGAG |
| Internal bar code: | CTACGCTGGACGCTAATGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2693 |
| LEAP-Seq percent confirming: | 97.0803 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 137 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACAGTGCACCATAGCGT |
| Suggested primer 2: | ATCAGGACATGCTGATGGCG |