| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056340 |
| Chromosome: | chromosome 14 |
| Location: | 2058549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621300 | (1 of 1) PTHR12375//PTHR12375:SF18 - RNA-BINDING PROTEIN LUC7-RELATED // LUC7-LIKE PROTEIN 3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTGCGAGCGGGAACCCTTGGTTGATCGTTTACCCGTCAACTCAAGGA |
| Internal bar code: | GTTTAAGCATGCTGTTACGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1748 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGCCTCAGCTCAGCTAC |
| Suggested primer 2: | CGACGACGGATACAGGTCAG |