Insertion junction: CLIP2.056344_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.056344
Chromosome:chromosome 1
Location:263437
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g001657 DAB1,DNAAF3,PF22 Dynein Assembly factor PF22; (1 of 1) PTHR22118//PTHR22118:SF14 - FAMILY NOT NAMED // DYNEIN ASSEMBLY FACTOR 3, AXONEMAL 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGGTTTTCCGGCCCTTCTGGCGCCGGGCGTCGAACACGCTCAACACA
Internal bar code:GAACACACTGCGCACTCTTGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:207
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTTCCGCTTGACTCCACCTT
Suggested primer 2:GTCGAGCGAGCATGAGATGA