| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056351 |
| Chromosome: | chromosome 16 |
| Location: | 1157364 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650250 | HFO39,HFO25 | (1 of 33) K11254 - histone H4 (H4); Histone H4 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACTCCCAGAATCTGAGCTTCCGGCATCTTCTACACCCGCCTATCCAG |
| Internal bar code: | ATACGGCAAGTAGTTCAGAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1733 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGCTGGAATGTGGTTTG |
| Suggested primer 2: | GACTCACACGCCCACTACAA |