| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056354 |
| Chromosome: | chromosome 16 |
| Location: | 4548689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671350 | ARS2 | (1 of 2) IPR012083 - Arylsulfatase; Periplasmic arylsulfatase 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTCATTAACCTACCCCAACACCACTCCAGGGGGGATCACGCACACGG |
| Internal bar code: | GAGTCAGACCGTGAAATGGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1964 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATGCCTAACACACACCC |
| Suggested primer 2: | TGCCGTTAAGTGCATCCTGT |