| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.056360 |
| Chromosome: | chromosome 5 |
| Location: | 3607608 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAAATAGCAATGCTGGAGCGAATTTCCTCGCAGGGCTCCGAACATGT |
| Internal bar code: | TTAAGATGTCGGGACATTCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1549 |
| LEAP-Seq percent confirming: | 91.1111 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTCCTTGGGCTTGTAG |
| Suggested primer 2: | GCGGTGCTTGGTTCAAACAT |