| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056373 |
| Chromosome: | chromosome 6 |
| Location: | 2941960 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273350 | NAT17 | N-acetyltransferase; (1 of 71) IPR016181 - Acyl-CoA N-acyltransferase | 3'UTR |
| Cre06.g273400 | TMC2 | putative tRNA (cytosine-5)-methyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGGACTACGGTGCATGCCATCGGCCATTCGCCACTTTCATGGTCACC |
| Internal bar code: | GGGGTGAGCGTGGGGCCGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 186 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCCTCTAATCCTGGTCGC |
| Suggested primer 2: | TGGAGGAGTGTCCCTATGGG |