| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056375 |
| Chromosome: | chromosome 6 |
| Location: | 337246 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251200 | IFT43,MOT41 | (1 of 1) PTHR33724//PTHR33724:SF1 - FAMILY NOT NAMED // INTRAFLAGELLAR TRANSPORT PROTEIN 43 HOMOLOG; Intraflagellar transport protein 43 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACTGCTGCATATCGGCACTGAGCCGGTGAACGAGTTCGCGGCCGTGCG |
| Internal bar code: | AATCAAGTGAGGAGGGGTATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2174 |
| LEAP-Seq percent confirming: | 90.7895 |
| LEAP-Seq n confirming: | 69 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 76 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTAGACCGAAGCCACCCT |
| Suggested primer 2: | AAGTAGGTTGCAACGGAGGG |