| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.056375 |
| Chromosome: | chromosome 6 |
| Location: | 337247 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g251200 | IFT43,MOT41 | (1 of 1) PTHR33724//PTHR33724:SF1 - FAMILY NOT NAMED // INTRAFLAGELLAR TRANSPORT PROTEIN 43 HOMOLOG; Intraflagellar transport protein 43 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATAAAGAGGCGCTAATGTGAAACGGCTTGTAAGTATGGCATTATCGA |
| Internal bar code: | TTTTTCAGGCATGTTTCTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2040 |
| LEAP-Seq percent confirming: | 49.2063 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTAGGTTGCAACGGAGGG |
| Suggested primer 2: | ATCTAGACCGAAGCCACCCT |