| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056382 |
| Chromosome: | chromosome 17 |
| Location: | 2574396 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716101 | POLL1 | (1 of 1) PF10391 - Fingers domain of DNA polymerase lambda (DNA_pol_lambd_f); DNA polymerase lambda | 3'UTR |
| Cre17.g716150 | SUMO90,SUM4 | Similar to Small ubiquitin-like modifier; (1 of 4) PF03171//PF11976 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy) // Ubiquitin-2 like Rad60 SUMO-like (Rad60-SLD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGCCTTTGACCGTCAACAAGACATCATCCTCAAGATCCAGCATTCCA |
| Internal bar code: | ATGGGTGTCTGAGGGGACCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4000 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTAATCAGGACCGAGCC |
| Suggested primer 2: | ATCTTTCGGGCCATCGGTTT |