Insertion junction: CLIP2.056419_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.056419
Chromosome:chromosome 4
Location:3209005
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g226400 (1 of 2) PTHR10252:SF25 - DNA POLYMERASE EPSILON SUBUNIT 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTTACACCCAAATGCCGCACACAGGAGATGTTCTTGGATGCGCTTGTG
Internal bar code:AGTTCGTGACGCTTAAGGTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:188
LEAP-Seq percent confirming:52.9412
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACATGGTTGTGCGACTG
Suggested primer 2:GCGCGTTGACAATTTGACCT