| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.056422 |
| Chromosome: | chromosome 12 |
| Location: | 7870482 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553600 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGATGATAGCGATCGTACCGACGTGCCGACGTGCCGGCAGGCCGCGGA |
| Internal bar code: | CCAGGGAGACCAGTAACATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 75.6098 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTCGTTTTCGTTGGTTT |
| Suggested primer 2: | AGAGGCGCAAGGAAACGTTA |