Insertion junction: CLIP2.056424_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.056424
Chromosome:chromosome 15
Location:5578229
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre46.g760947 (1 of 7) IPR022772 - von Hippel-Lindau disease tumour suppressor, beta/alpha domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTTCCCACACGCACACTCCCACGCACACACACGTACCCCCCACAGGC
Internal bar code:ACAAAATCAAGACCATTTGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:203
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGCAAGGTTTGGGAGTTT
Suggested primer 2:CCACGTTGCACAAGGACTTG