Insertion junction: CLIP2.056447_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.056447
Chromosome:chromosome 10
Location:6619182
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g465900 CDKA1 Cyclin-dependent kinase; (1 of 4) K02206 - cyclin-dependent kinase 2 (CDK2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGATGCAATCGCCCACCCAGCAACTGCTGCTGCTAGGGCACCAGCACT
Internal bar code:CCGACGGTTCACAGACAGGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:938
LEAP-Seq percent confirming:48.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTTGGTGAGGCAGTTTTG
Suggested primer 2:TGATCATGCAACACGTCCCA