Insertion junction: CLIP2.056450_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.056450
Chromosome:chromosome 3
Location:1842045
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g154400 MFT15 Major facilitator superfamily transporter; (1 of 2) PTHR23121:SF9 - SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAACTAAACTGAATCCACTCAGGTCGAGCAGGTCCACGCAGCTGGTGTA
Internal bar code:AGGGAATGATGTCGGTTGCCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4321
LEAP-Seq percent confirming:95.3125
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAACCTGCGTGGCATATG
Suggested primer 2:CGGGCCTTTTGGTTCTGTTG