Insertion junction: CLIP2.056460_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.056460
Chromosome:chromosome 16
Location:8006694
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g692228 MARS1 Mutant Affecting Retrograde Signaling 1; (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACATTTGAGTCATCAACAAGTTACCCTAGCTTATTGAGGTTCCTCTGGC
Internal bar code:ACTGTCGGTATCGGCAGAGCAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3241
LEAP-Seq percent confirming:97.8261
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTCGTGCGTCAACTTATC
Suggested primer 2:CACGCATGCATGTCAGATGG