Insertion junction: CLIP2.056462_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.056462
Chromosome:chromosome 16
Location:7309876
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre16.g802006 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTCGGCATCGCACACACGATGTCATTGCGGGCTTCGGCGTCACGCTT
Internal bar code:CGTAGGGGAGAAGGGGTACTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:713
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACGGACTCGCTAGGGTAGT
Suggested primer 2:CTGCGCACTGAATCGACATG