Insertion junction: CLIP2.056463_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.056463
Chromosome:chromosome 16
Location:3909950
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g689250 GTR23 (1 of 1) PF04564//PF06701 - U-box domain (U-box) // Mib_herc2 (MIB_HERC2); Putative ubiquitin-protein ligase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGGCTGTTGCCAAAACGACGGGTATTCAGTGCGTAACACCGTGTTCAC
Internal bar code:ACGGGACCGTCAACATCATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:774
LEAP-Seq percent confirming:55.5556
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CTGAAGCTGGGGTGTTGCTA
Suggested primer 2:GGCTATACGGTCTTCCCACG