| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056466 |
| Chromosome: | chromosome 12 |
| Location: | 9183915 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543301 | CSV11 | Chlamydomonas-specific family protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCACCGGTGAAAAGCTGATGGCACTCGCTGTGCTCGCTCTGCTCAGC |
| Internal bar code: | CGTAGAAGAGCCTGTCCCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1174 |
| LEAP-Seq percent confirming: | 51.6129 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGACACAAGGCGACCTG |
| Suggested primer 2: | CTGGGCATTCAGGAATGGGT |