| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.056480 |
| Chromosome: | chromosome 5 |
| Location: | 3029522 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g231050 | (1 of 1) K10636 - autocrine motility factor receptor (AMFR, GP78) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACGACGGGCCCGGTCCCGCGCCGCCGCCGCCGCCGCCTGCGGCGGCG |
| Internal bar code: | TGGGTTTGCGTTTTGAAGAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTCAAACGACGTCAGA |
| Suggested primer 2: | CCAAACATCCCTGCATCCCT |