| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.056493 |
| Chromosome: | chromosome 3 |
| Location: | 573596 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145307 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTGCTGTTGCTCGTTGTCATGCAGCAGTCGCCCTTGCTTTAAGCCCT |
| Internal bar code: | CTCGCGTTGAGTGCTGACTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 52.9412 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACTACGCGAATGTACGG |
| Suggested primer 2: | TCAGTAAACTCGGTCGGCAC |