Insertion junction: CLIP2.056499_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.056499
Chromosome:chromosome 17
Location:71174
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g696350 (1 of 1) PTHR12454:SF11 - PROTEIN Y57A10A.10 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCAACAAGTGTTCGCAAAAACATTGTAACGGAGTGCATACTCTCAAA
Internal bar code:GTAGCGGTCGGACTAGGAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:4709
LEAP-Seq percent confirming:97.7528
LEAP-Seq n confirming:87
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCAACAGCTGCTACACA
Suggested primer 2:TCTTGGAGTCACCTTCGTGC