| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056502 |
| Chromosome: | chromosome 5 |
| Location: | 3112256 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755797 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCCCGGACCCCCGTGGTGGTGTCCCGCCGAACCGCGACCCCAACCTGT |
| Internal bar code: | GGCAGCCATGCCGTACTAATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 196 |
| LEAP-Seq percent confirming: | 5.81395 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGGGTGCTTGACAAGT |
| Suggested primer 2: | GGAACTCCTCCACACACGAG |