| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.056514 |
| Chromosome: | chromosome 10 |
| Location: | 6529074 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g465400 | (1 of 1) IPR026305 - Negative elongation factor A | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGCGGCGCTGCTGGCGGCGGCGCGGGCGACGGCGCCGCCGCAGCTGC |
| Internal bar code: | ACATGTTGGAGCAATATTATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGATACACACACGCACA |
| Suggested primer 2: | TGGTGAATGGAGGGACATGC |