| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056540 |
| Chromosome: | chromosome 2 |
| Location: | 5629262 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115550 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCATTGGCCAGGACTGCACAGTGTGGACGTACACGTCCGGAGGCGGCA |
| Internal bar code: | ACAGCACGGACCGGTAGGACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3972 |
| LEAP-Seq percent confirming: | 96.1538 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAACGCCATACCTAGCTA |
| Suggested primer 2: | ATGCGGGTTAAAGGTCCAGG |