| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.056545 |
| Chromosome: | chromosome 16 |
| Location: | 3972350 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689700 | HPR5 | (1 of 1) 1.1.1.272 - D-2-hydroxyacid dehydrogenase (NADP(+)); Hydroxypyruvate reductase 5 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCTTTGCTTTGCTTTCACCTCGTTGCCCATAGAACTGCCTTCATAATTG |
| Internal bar code: | ACGGTCGCGTTCATCGTATAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2061 |
| LEAP-Seq percent confirming: | 13.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCTTTATCCGCTTGC |
| Suggested primer 2: | AGCAAGTTCACTGAGGACCG |