| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056560 |
| Chromosome: | chromosome 6 |
| Location: | 2817149 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g271900 | (1 of 1) 1.1.99.37 - Methanol dehydrogenase (nicotinoprotein) / Nicotinoprotein methanol dehydrogenase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCCCTTTGGTCCCATGCTGCCTTGCAGGCACTCGCTCGCTCTTCGACT |
| Internal bar code: | GTTTCAATGCGCAGTTTAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1160 |
| LEAP-Seq percent confirming: | 94.7368 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCATCAACCCCTCACAT |
| Suggested primer 2: | CAATGCGTGCGCTAGTTACC |