| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.056622 |
| Chromosome: | chromosome 13 |
| Location: | 4455104 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602650 | (1 of 1) PTHR12231//PTHR12231:SF150 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 11, CHLOROPLASTIC | 3'UTR | |
| Cre13.g602700 | SPA1 | Part of COP1/SPA complex E3 ubiquitin ligase complex; (1 of 1) K16240 - protein suppressor of PHYA-105 1 (SPA1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGCAGCAACGATGCTCGACGGCAGACTTATAGACGCCGGCGTGCCGT |
| Internal bar code: | GAGTAACCCTGCATTCGGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 10.7143 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTATGCCATGTTCCCCCA |
| Suggested primer 2: | CAAGGGAAAGCGTGTGTGTG |