| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.056641 |
| Chromosome: | chromosome 4 |
| Location: | 3859784 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230144 | SPT1 | Component of serine palmitoyltransferase; (1 of 1) PTHR13693//PTHR13693:SF3 - CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE // LD36009P | 3'UTR |
| Cre04.g230242 | MOT40 | (1 of 1) IPR007914//IPR015422 - Uncharacterised protein family UPF0193 // Pyridoxal phosphate-dependent transferase, major region, subdomain 2; Predicted protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCGACGCGAGAGCAGAGTAGGGGTTGCTTAATGCGGCCAGGTGTTTG |
| Internal bar code: | CCATTACGGAGATAAAACTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 77.9412 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCCCTGAGTCATGACAGC |
| Suggested primer 2: | TTTTGGCGCGTGAGTTTCAG |