| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056654 |
| Chromosome: | chromosome 12 |
| Location: | 756542 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492550 | (1 of 781) IPR000104 - Antifreeze protein, type I | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGTCCGTGCCTGCCTGCCTGCCTGCGTTGACAAAAAGTGCCAAACTG |
| Internal bar code: | TGAATTCCGGATGTTTATCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4413 |
| LEAP-Seq percent confirming: | 98.2759 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGGCCCGGATGATTATC |
| Suggested primer 2: | CATGGTTGGGCAGGCTCTTA |