| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.056657 |
| Chromosome: | chromosome 10 |
| Location: | 5220697 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g456050 | AGG6 | (1 of 4) K03809 - NAD(P)H dehydrogenase (quinone) (wrbA); Aggregation 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAACGAGAAAGCTCTCTGCCCTCTCCAGTTACACCAGACCGCTCGGCTG |
| Internal bar code: | GTGACTCGGGTGGAATGGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1714 |
| LEAP-Seq percent confirming: | 92.5926 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTTGTCGTGCTGCAAC |
| Suggested primer 2: | GCAACTTCGTTCACCACCAC |