| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056661 |
| Chromosome: | chromosome 13 |
| Location: | 4454648 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g602650 | (1 of 1) PTHR12231//PTHR12231:SF150 - CTX-RELATED TYPE I TRANSMEMBRANE PROTEIN // RHODANESE-LIKE DOMAIN-CONTAINING PROTEIN 11, CHLOROPLASTIC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTAGCGGATCTGGCCTGCTGGTTGGAGGCACCGCATTTGCTAACAGC |
| Internal bar code: | TCCCACCCGACACCCCGAAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1054 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCCCACCTCATCATCAAC |
| Suggested primer 2: | CAAGGGAAAGCGTGTGTGTG |