| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056663 |
| Chromosome: | chromosome 13 |
| Location: | 2706415 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581700 | (1 of 6) 2.7.7.84 - 2'-5' oligoadenylate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGACACAGCGCCTCAAACCAATAATCAAATCACGGCACGCCGTCACGT |
| Internal bar code: | TACACCCAGTGGTGGAGTGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3535 |
| LEAP-Seq percent confirming: | 58.4416 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 64 |
| LEAP-Seq n unique pos: | 154 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCAGCAGGTGCAATGCA |
| Suggested primer 2: | AGCGTGTGTTTACCGTACGT |