| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056675 |
| Chromosome: | chromosome 9 |
| Location: | 5481575 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408851 | (1 of 2) 3.4.11.9 - Xaa-Pro aminopeptidase / X-Pro aminopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACCAACGAGGGGGTGTCAAGGATTGTTGGAAAATGTGATAGAGGATG |
| Internal bar code: | GCGTCAGCGTTTTTAGTGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 84.2105 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTGGAGACGCTTGGTAC |
| Suggested primer 2: | TCGTAATTGTGTCCCCGCAA |