Insertion cassette: | CIB2 |
Side of cassette: | 5' truncated? |
Strand: | - |
Strain: | CLIP2.056688 |
Chromosome: | chromosome 10 |
Location: | 3104017 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g441450 | (1 of 1) K14786 - protein KRI1 (KRI1) | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTAGTCTTTCGGCTTCGGGTGTAGACTAGTTACTTGGAGCACCGACA |
Internal bar code: | TGGCCTCTCCAAACTGATTTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3025 |
LEAP-Seq percent confirming: | 96.2963 |
LEAP-Seq n confirming: | 26 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGGAGGCATGGATGGACAAC |
Suggested primer 2: | ACCCCAACCTTTGCTCCAAA |