Insertion junction: CLIP2.056690_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.056690
Chromosome:chromosome 1
Location:6217363
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCAGGGGGTTTTCACCCCAGCCAAACCCGCACGCACAGGAGCCTGCAC
Internal bar code:TTGATTTAGGGTCCCTTTTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3074
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ATAAGGATTTTTCGCGCGGC
Suggested primer 2:GAGGAGGCCAATCCAGCTTT