Insertion junction: CLIP2.056709_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.056709
Chromosome:chromosome 2
Location:2876617
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGTTGTAACAACAGTTACCCGCGAAATGCCCACTCAATCGAGGGGGTA
Internal bar code:TGATTGCGAATAAGCTATATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4418
LEAP-Seq percent confirming:97.7273
LEAP-Seq n confirming:43
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CATTGCTCCCGAGGGACTTT
Suggested primer 2:AGTGGACCGCTCATGTTCTG