| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.056712 |
| Chromosome: | plastome |
| Location: | 89944 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802300 | rps18,ChreCp036,2717007 | (1 of 2) PF01084 - Ribosomal protein S18 (Ribosomal_S18); 30S ribosomal protein S18 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACGACGATTATAAAGAGTTTTTTCAGGTTTTTCTTTAAGAACAATTAC |
| Internal bar code: | ATAGATCGGGCATTGGTTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 5.26316 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAATTGCAGCCCCTCAGT |
| Suggested primer 2: | GAAGGGGAAGGACGTCAGTG |