| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.056719 |
| Chromosome: | chromosome 12 |
| Location: | 7119096 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559700 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAAGGGCAGCGCACCGACAGCAGCCAAACGGGCTGAGAGTGGAGAACG |
| Internal bar code: | TGTCACCTTGTTAACAGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2132 |
| LEAP-Seq percent confirming: | 20.202 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 99 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCACGTTCCAAACCTGTT |
| Suggested primer 2: | GCCTTGTCACGTTCCCAAAC |