| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.056721 |
| Chromosome: | chromosome 17 |
| Location: | 3060932 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720183 | (1 of 1) K08998 - hypothetical protein (K08998) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATACACCTACTGCCGCGACGCCGGCTAAGGTGGCATGTAATCCAGATAG |
| Internal bar code: | TCGGGGATAGGTTATTCTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1712 |
| LEAP-Seq percent confirming: | 85.567 |
| LEAP-Seq n confirming: | 83 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCTTGTCCAGAGCGGTC |
| Suggested primer 2: | GCTAGGTTGTGAGTGCCTGT |