Insertion junction: CLIP2.056722_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.056722
Chromosome:chromosome 6
Location:5665140
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g287850 (1 of 781) IPR000104 - Antifreeze protein, type I 3'UTR
Cre06.g287900 (1 of 1) PF10067 - Predicted membrane protein (DUF2306) (DUF2306) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAGTCCTAAACGCTCCCGATACCGGTATCGCGCATATGGTGCGAAGCC
Internal bar code:TTCTGTTCTGGACTATTCACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:3490
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGGGAGCATGCACACAT
Suggested primer 2:TTTGCGGCAAGTTGGTGATG