Insertion junction: CLIP2.056724_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.056724
Chromosome:plastome
Location:159959
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802323 2717034,atpB,ChreCp058 (1 of 1) K02112 - F-type H+-transporting ATPase subunit beta (ATPF1B, atpD); ATPase beta chain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACGGAAAATGTTATCAATGAAGAATAATACGTCTTGTTTGTTAACATC
Internal bar code:TAGGTTGAGAAAGGACATTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:130
LEAP-Seq percent confirming:8.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGTCTTTTCGGTGGTGCT
Suggested primer 2:ACTCGCACGTTTCCTTTAGT