Insertion junction: CLIP2.056744_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.056744
Chromosome:chromosome 2
Location:7368068
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g391912 (1 of 24) PTHR10641 - MYB-LIKE DNA-BINDING PROTEIN MYB 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTCAAATCCCCAGTAACTGTGGCATTTGGAGCAGTAGCAACATAGCA
Internal bar code:TGAAAAACTTGTAGACAGAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:2114
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GAGTGGCTGCTTCTGTGGAT
Suggested primer 2:AGGACTGATGACAGCGCAAA