| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056745 |
| Chromosome: | chromosome 12 |
| Location: | 4680843 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g522450 | SEC31 | (1 of 1) K14005 - protein transport protein SEC31 (SEC31); COP-II coat subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGTAAGGCATTATGTCGCATAGCCAAGGTACTTGAATTGCCGAGCTT |
| Internal bar code: | CTTGGGACTTGCTTGAACGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3993 |
| LEAP-Seq percent confirming: | 75.6757 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCATCCGCCCTCTTCTGG |
| Suggested primer 2: | AATGATCCCCCACAGCCAAG |