| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.056761 |
| Chromosome: | chromosome 17 |
| Location: | 4043403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTTATCGATTACTGTACTCTCAAGTCTAGGTCTGTCGTGCCTGGTGCG |
| Internal bar code: | CAGAATGCTTTGCTCTTAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1670 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTGAGAAAAGCCGTGGAC |
| Suggested primer 2: | TCGGCACACGGATTGTAACA |