| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.056780 |
| Chromosome: | chromosome 17 |
| Location: | 5201063 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g736950 | (1 of 1) PF11265 - Mediator complex subunit 25 von Willebrand factor type A (Med25_VWA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCATCTTGACACGATATATCATACCTTGCGGATTGCCAAGTTGCGCAGT |
| Internal bar code: | GGTGAACGACTATGGTCATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1938 |
| LEAP-Seq percent confirming: | 65.625 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGGGCGCTTGCTTGATG |
| Suggested primer 2: | ACGCCATCCTATCATCAGCG |